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Background: Glioblastoma (GBM) is an aggressive brain tumor giving a poor prognosis with the current treatment options. The advent of chimeric antigen receptor (CAR) T-cell therapy revolutionized the field of immunotherapy and has provided a new set of therapeutic options for refractory blood cancers. In an effort to apply this therapeutic approach to solid tumors, various immune cell types and CAR constructs are being studied. Notably, macrophages have recently emerged as potential candidates for targeting solid tumors, attributed to their inherent tumorinfiltrating capacity and abundant presence in the tumor microenvironment. Materials and methods: In this study, we developed a chemically defined differentiation protocol to generate macrophages from human pluripotent stem cells (hPSCs). A GBM-specific CAR was genetically incorporated into hPSCs to generate CAR hPSC-derived macrophages. Results: The CAR hPSC-derived macrophages exhibited potent anticancer activity against GBM cells in vitro. Conclusion: Our findings demonstrate the feasibility of generating functional CAR-macrophages from hPSCs for adoptive immunotherapy, thereby opening new avenues for the treatment of solid tumors, particularly GBM. 

We propose an accessible indoor navigation application. The solution integrates information of floor plans, Bluetooth beacons, Wi-Fi/cellular data connectivity, 2D/3D visual models, and user preferences. Hybrid models of interiors are created in a modeling stage with Wi-Fi/ cellular data connectivity, beacon signal strength, and a 3D spatial model. This data is collected, as the modeler walks through the building, and is mapped to the floor plan. Client-server architecture allows scaling to large areas by lazy-loading models according to beacon signals and/or adjacent region proximity. During the navigation stage, a user with the designed mobile app is localized within the floor plan, using visual, connectivity, and user preference data, along an optimal route to their destination. User interfaces for both modeling and navigation use visual, audio, and haptic feedback for targeted users. While the current pandemic event precludes our user study, we describe its design and preliminary results.